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We have previously described the generation of monoclonal antibodies (MAbs) against Trichophyton rubrum mannoprotein (38 kDa), a glycoprotein of the cell wall of dermatophytes. Some of these MAbs were used to determine the presence of carbohydrate epitopes of the 38 kDa antigen on the surface of T. rubrum. The 38 kDa mannoprotein was isolated by immunoadsorption and labelled with 125I using the lactoperoxidase technique. The epitope specificity of the MAbs was tested on different assays: a haemagglutination inhibition test; binding to the carbohydrate moiety of the mannoprotein; inhibition of the interaction of the mannoprotein with antibody; binding to the 38 kDa glycoprotein antigen isolated from T. rubrum and inhibition of the haemagglutination activity of the MAb. 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